Characterization of autoantibodies to 60-kD SS-A, 52-kD SS-A and SS-B in Japanese lupus mothers with neonatal lupus syndrome.

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52 kD Ro/SS-A localizes to punctate structures in the cytoplasm of epithelial cells.

Autoantibodies directed against 52 kD and 60 kD Ro/SS-A are frequently found in the sera of patients with lupus erythematosus and Sjögren's syndrome-related disorders. Their location in the cell is subject to continuous debate in literature. It has been postulated that 52 kD Ro (52 Ro) co-localizes with the 60 kD Ro autoantigen in the nucleus, while others demonstrated that 52 Ro is primarily c...

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A 52-kD protein is a novel component of the SS-A/Ro antigenic particle

Anti-SS-A/Ro autoantibodies are found in the sera of patients with Sjogren's syndrome (SS) and SLE. In the course of analyzing 61 SS patients for their autoantibody profiles, we found that 42 were positive for anti-SS-A by double diffusion in agarose and demonstrated precipitin lines identical to that produced by a prototype anti-SS-A serum. Further analysis of these SS-A antibody-positive sera...

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Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen.

Serum SS-A/Ro autoantibodies are commonly found in patients with Sjogren's syndrome, systemic lupus erythematosus, neonatal lupus, and subacute cutaneous lupus. Two proteins of 60 and 52 kD have been described as targets for these autoantibodies. To define the 52-kD component unambiguously, cDNA clones were isolated from human HepG2 and MOLT-4 cell cDNA libraries. The identity of cDNA was estab...

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Tear fluid measurement of anti-SS-A and anti-SS-B antibody in anti-SS-A and anti-SS-B seronegative Sjögren's syndrome patients.

Sirs, In the US/European Consensus Group (EUCG) classification criteria for Sjögren’s syndrome (SS) objective items, such as focal sialadenitis and serum presence of antiSS-A and anti-SS-B antibodies (anti-SS-A, anti-SS-B), are emphasized (1). In the sera of approximately 20% of SS patients no anti-SS-A or anti-SS-B can be detected at the time of diagnosis. However, in these ‘seronegative’ pati...

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Prevalence and clinical relevance of 52-kDa and 60-kDa Ro/SS-A autoantibodies in Japanese patients with systemic sclerosis.

OBJECTIVE To determine the prevalence of 52-Kda and 60-Kda Ro/SS-A autoantibodies in serum samples from Japanese patients with systemic sclerosis (SSc). METHODS Serum samples from 263 Japanese patients with SSc were examined by double immunodiffusion and enzyme linked immunosorbent assay (ELISA). RESULTS By double immunodiffusion, 29 serum samples from patients with SSc were found to posses...

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ژورنال

عنوان ژورنال: Japanese Journal of Clinical Immunology

سال: 2001

ISSN: 0911-4300,1349-7413

DOI: 10.2177/jsci.24.291